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human enzyme linked immunosorbent assay elisa elabscience  (Elabscience Biotechnology)


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    Elabscience Biotechnology human enzyme linked immunosorbent assay elisa elabscience
    Human Enzyme Linked Immunosorbent Assay Elisa Elabscience, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human enzyme linked immunosorbent assay elisa elabscience  (Elabscience Biotechnology)
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    Melatonin level declines with aging in human bone marrow and mouse plasma. (A) Representative images of lysosomal β‐galactosidase staining in bone marrow stromal cells (BMSCs) from donors with different ages. Scale bar, 50 μm. (B) Quantification of the percentage of β‐galactosidase positive BMSCs in three groups ( n = 12). Nine random vision fields with 200 × magnification were analysed. (C) Melatonin levels in the bone marrow plasma of donors under 45 years old ( n = 15) or over 60 years old ( n = 24) measured by ELISA. (D) Toluidine Blue O staining of the trabecular bone near the distal femoral metaphyseal region from adult (4 months) and aged mice (24 months). (E) Representative three‐dimensional trabecular architecture examined by a micro‐CT scan of the trabecular bone near the distal femoral metaphyseal region from adult and aged mice. Region of interest (ROI), 1 mm length starting from .5 mm proximal to the growth plate consisting of 140 slices. (F) Quantifications of the trabecular bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), trabecular separation (Tb.Sp) and mean cortical area fraction (B.Ar/T.Ar) ( n = 9 mice per group). (G) Melatonin level in the peripheral blood of adult and aged mice. n = 9 mice per group. Data are mean ± s.e.m. p values are determined by unpaired two‐sided t ‐tests with Welch's correction

    Journal: Clinical and Translational Medicine

    Article Title: Melatonin enhances osteoblastogenesis of senescent bone marrow stromal cells through NSD2‐mediated chromatin remodelling

    doi: 10.1002/ctm2.746

    Figure Lengend Snippet: Melatonin level declines with aging in human bone marrow and mouse plasma. (A) Representative images of lysosomal β‐galactosidase staining in bone marrow stromal cells (BMSCs) from donors with different ages. Scale bar, 50 μm. (B) Quantification of the percentage of β‐galactosidase positive BMSCs in three groups ( n = 12). Nine random vision fields with 200 × magnification were analysed. (C) Melatonin levels in the bone marrow plasma of donors under 45 years old ( n = 15) or over 60 years old ( n = 24) measured by ELISA. (D) Toluidine Blue O staining of the trabecular bone near the distal femoral metaphyseal region from adult (4 months) and aged mice (24 months). (E) Representative three‐dimensional trabecular architecture examined by a micro‐CT scan of the trabecular bone near the distal femoral metaphyseal region from adult and aged mice. Region of interest (ROI), 1 mm length starting from .5 mm proximal to the growth plate consisting of 140 slices. (F) Quantifications of the trabecular bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), trabecular separation (Tb.Sp) and mean cortical area fraction (B.Ar/T.Ar) ( n = 9 mice per group). (G) Melatonin level in the peripheral blood of adult and aged mice. n = 9 mice per group. Data are mean ± s.e.m. p values are determined by unpaired two‐sided t ‐tests with Welch's correction

    Article Snippet: Melatonin level in bone marrow plasma was measured with human MT (Melatonin) Enzyme Linked Immunosorbent Assay (ELISA) Kit (Elabscience, Cat: E‐EL‐H2016c, Wuhan, China) accordingly.

    Techniques: Clinical Proteomics, Staining, Enzyme-linked Immunosorbent Assay, Micro-CT